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PediTree installs and runs perfectly well under Windows 7, both 32-bit and 64-bit versions. It should be installed in the usual way, preferably from a recent CD supplied by Pedigree Software. Do not be tempted to install it in one of the Program files directories, as these have special security settings that can produce difficulties. Simply use C:\pedigree (or a similar root folder).
You can, if you wish, place your databases in a different location under C:\Users… When transferring your databases onto the new system, use a USB memory-stick or a CD, so that you do not transfer the Windows security settings as well. The old security settings may be incompatible with the new system. These are transferred if you use an external NTFS disk-drive or some transfer programs and can produce difficulties with unreadable databases.
The various utility programs (Database Utilities, GedUtils, PedSpecs and PedClone) also work under both 32- and 64-bit versions.
Pedigree for DOS will install and work under 32-bit Windows 7, but not under the 64-bit versions. If you must have it on a 64-bit system, then it can be run using a free utility called DosBox, as shown in dosbox.
The appearance of a message-box like the above with the same heading is often due either to a missing file or to a fault in your database. From PediTree version 3.330 (February 2010), missing or inaccessible files should be detected before DB32 is involved, so these messages are probably due to a database fault (the example above is one such).
The quick fix is to restore your database from a recent back-up copy. Alternatively, you may prefer to repair the database, if possible. Instructions for this are available here.